摘要
目的：探讨充血性心力衰竭（ＣＨＦ）患者心肌细胞内ＡＴＰ酶、钠、钾、钙、镁、脂质过氧化的变化及其相互关系。方法：以红细胞为模型，采用原子吸收分光光度法测定３０例ＣＨＦ患者红细胞内钠、钾、钙、镁离子浓度（［Ｎａ＋］ＲＢＣ、［Ｋ＋］ＲＢＣ、［Ｃａ２＋］ＲＢＣ、［Ｍｇ２＋］ＲＢＣ），并且同步测定了红细胞内ＡＴＰ酶、超氧化物歧化酶（ＳＯＤ）的活性及血清脂质过氧化物（ＬＰＯ）的含量，并以３０例正常人作对照。结果：①ＣＨＦ患者ＬＰＯ、［Ｃａ２＋］ＲＢＣ、［Ｎａ＋］ＲＢＣ均显著高于正常人（Ｐ均＜０．０５），红细胞Ｎａ＋Ｋ＋ＡＴＰａｓｅ、Ｃａ２＋Ｍｇ２＋ＡＴＰａｓｅ、ＳＯＤ、［Ｋ＋］ＲＢＣ、［Ｍｇ２＋］ＲＢＣ均显著低于正常人（Ｐ均＜０．０５），其变化的趋势与心力衰竭程度相平行。②Ｎａ＋Ｋ＋ＡＴＰａｓｅ与ＬＰＯ之间呈负相关（ｒ＝－０．７１２５，Ｐ＜０．０５）；Ｃａ２＋Ｍｇ２＋ＡＴ－Ｐａｓｅ与ＬＰＯ之间呈负相关（ｒ＝－０．７４３２，Ｐ＜０．０１），与ＳＯＤ之间呈正相关（ｒ＝０．７１９０，Ｐ＜０．０５）；［Ｋ＋］ＲＢＣ与Ｎａ＋Ｋ＋ＡＴＰａｓｅ之间呈正相关（ｒ＝０．５２００，Ｐ＜０．０５）；Ｃａ２＋Ｍｇ２＋ＡＴＰａｓｅ与［Ｃａ２＋］?
Objective:To research the changes on ATPase,Na +,K +,Ca 2+ ,Mg 2+ and lipid peroxide(LPO) in myocardial cell and the relationship among them in 30 patients with congestive heart failure (CHF) and 30 cases of health.Methods:The red blood cell (RBC) was used as model for study.The concentration of Na +、K +、Ca 2+ and Mg 2+ in RBC were measured with atomic absorption spectrophotometry.In the meantime,the activity of superoxide dismutase(SOD) and ATPase in RBC,the concentration of serum LPO were measured simultaneous.Results:①The levels of LPO、[Ca 2+ ] and in RBC in patients with CHF were significantly higher than health (all P <0 05),while the levels of Na +K +ATPase,Ca 2+ Mg 2+ ATPase,SOD, and [Mg 2+ ] in RBC were lower obviously than health(all P <0 05).The more severe of CHF the abnormal were them.②The level of LPO was negatively related to Na +K +ATPase( r =-0 7125, P <0 05),the LPO was negatively related to Ca 2+ Mg 2+ ATPase( r =-0 7432, P <0 01),the SOD was positively related to Ca 2+ Mg 2+ ATPase( r =0 7190, P <0 05),the activity of Ca 2+ Mg 2+ ATPase was negatively related to Ca 2+ ( r =-0 6300, P <0 05)and positively related to Mg 2+ ( r =0 5000, P <0 05),the content of K + was positively related to Na +K +ATPase( r =0 5200, P <0 05).Conclusions:These data showed that lipid peroxidation may depress the ATPase activity and thus cause the changes of cation which is an important pathogenesis of CHF.
