摘要
目的观察在体外实验中,包含有重复序列TTAGGG的寡核苷酸(ODN_(A151))对小鼠脾淋巴细胞分泌Th1、Th2型细胞因子的影响。方法分离小鼠睥淋巴细胞,根据分组加入伴刀豆蛋白A(ConA)以及不同浓度的ODN_(A151)或对照的ODN_(1612),培养72 h后采用夹心法ELISA测定上清中的IFN_γ和IL-4浓度。结果ODN_(A151)抑制淋巴细胞培养上清的IFNγ浓度,并升高IL-4的浓度,降低IFN_γ与IL-4的比值,并且ODN_(A151)的作用呈剂量依赖关系;而对照的ODN_(1612)对细胞因子水平无影响。结论在非抗原特异性、多克隆刺激剂(ConA)作用下,ODN_(A151)对小鼠睥淋巴细胞的Th1/Th2平衡有调节作用,既抑制Th1分化又促进Th2分化。
Objective To explore the effect of oligodeoxynucleotides （TTAGGG） on the cytokines secreted by splenetic lymphocytes of BALB/c mice. Methods Firstly, separated splenetic lymphocytes of BALB/c mice; after cultivation of 72 hours with concanavalin A （ConA） and several concentration of （1 μM, 5 μM, 10 μM）ODNA151 or ODN1612（10 μM）, then detected IFN γ/and IL-4 in supernatant by ELISA. Results ODNA151 but not ODN1612 significantly inhibited IFNγ/and increased IL-4 （P 〈 0.01 ）, accordingly down-regulated ratios of IFNT/IL-4; This effect was linear correlated on the concentration of ODNA151. Conclusion On stimulation of ConA, ODNA151 regulated differentiation of Th lymphocytes and skewing Th1/ Th2 balance toward Th2 type eytokine production.
出处
《中国分子心脏病学杂志》
CAS
2007年第1期18-20,共3页
Molecular Cardiology of China
基金
受国家自然科学基金资助(基金批准号:30600234)
