杭白菊同源四倍体的诱导和鉴定

The Inducement and Identification of the Polyploid of Chrysanthemum Morifolium Ramat.

采用正交试验设计方法,进行了杭白菊试管苗快速繁殖技术的优化,同时进行了组织培养条件下杭白菊同源四倍体诱导与鉴定技术的研究。结果表明,诱导愈伤组织的最优外植体为叶片,最适培养基为MS+KT(激动素)2.0 mg/L+NAA(萘乙酸)0.2 mg/L;确定了杭白菊最佳繁殖培养基为MS+BA(6-苄基嘌呤)0.2 mg/L+IAA(吲哚乙酸)0.05 mg/L+NAA0.2 mg/L;最佳生根培养基为1/2MS+IAA 0.2 mg/L+ABT(生根粉)0.3 mg/L。在诱导四倍体的试验中,秋水仙碱浓度对存活率有显著影响,诱导杭白菊同源四倍体的最佳条件是在2%二甲基亚砜和浓度为0.2%的秋水仙碱中浸泡36 h,诱导率高达13.33%。通过根尖细胞染色体鉴定,共获得11个同源四倍体株系。

The rapid-propagation system of Chrysanthemum morifolium has been established. The results showed that the leaves are the best explant to induce callus and the MS ＋KT2.0 mg/L＋NAA 0. 2 mg/L is the best medium. The results indicated that the strong and green clump-buds could be induced on the MS media containing BA0.2 mg/L,IAA0.05 mg/L and NAA0. 2 mg/L. The strong root was induced on the 1/2MS, IAA0. 2 mg/L and ABT0. 3 mg/L; The technology of inducing autotetraploid of Chrysanthemum morifoliurn was studied in vitro. The results indicated that autotetraploid can be induced by immersing the buds in 0. 2 % colchicines solution supplemented with 2 % DMSO. This method is proved to be the best way to induce autotetraploid with inducing ratio as high as 13.33%. 11 lines of autotetraploid lines were identified by optimized chromosome deterrninmation method. It has laid the basis for further selecting the varieties of Chrysanthemum rnorifoliurn with good quality and high yield.