金鱼草高效植株再生体系的建立

Establishment of High Efficient Regeneration System of Antirrhinura majus L.

[目的]探索利用组织培养技术对优良品种的金鱼草进行快速繁殖的研究。[方法]以金鱼草无菌苗不同部位为外植体,在MS+2.0mg/LBA+0.2mg/LNAA的培养基上进行再生频率比较。[结果]结果表明,下胚轴的再生率最高;把下胚轴再生的芽接种到增粗培养基上进行再生芽的诱导,可以得到100%的再生率。[结论]该研究为金鱼草的高效转化提供了试验依据。

[Objective] Rapid regeneration of good variety A ntirrhinura majus L was studied by tissue culture technology. [Method] Different explants of A ntirrhinura majus L was cultured on MS＋2.0 mg/L BA＋0.2 mg/L NAA and their regeneration frequencies were compared. [Result] Result showed that hypocotyls had the highest regeneration frequency. The bud of hypocotyls was transformed into regeneration medium and its regeneration frequency could reach 100 %. [Conclusion] This paper provided references for the high efficient regeneration study of A ntirrhinura ,majus L.