骨骼肌肌纤维钙火花检测中的伪彩色增强

Pseudo-color enhancement in calcium sparks detection of skeletal muscle fiber

利用激光扫描共聚焦显微镜,对经Flou-3AM染色的、人工剥离的蟾蜍骨骼肌肌纤维进行连续扫描分析,捕获电刺激后骨骼肌肌纤维中发生的钙火花现象,并通过对检测结果进行伪彩色处理,将灰度图像转换为彩色图像,从而提高了检测结果的直观性,增强了对钙火花的表达能力。

Fluorescence probe Fluo-3AM can be luminary in confocal microscope after combining Ca2＋, So nomadic Ca2＋ in cytoplasm can be measured and tested by this way.Some confocal laser scan images are obtained after calcium in muscle fibers signed with Fluo-3AM. Then mapping function is used to convert the gray level image into colorful image by pseudocolor enhancement theory in digital image.Compared to the original gray level image, the processed pseudo-color image could be more easily distinguished.So the methods had a great value in Ca2＋ detection fields.