大孔吸附树脂纯化辣木叶总皂苷工艺研究

Studies on the process of Moringa Oleifera saponins purified with macroporous adsorption resin

研究大孔吸附树脂纯化辣木叶总皂苷的工艺条件和参数。以洗脱率、精制度等为指标,考察大孔吸附树脂对辣木叶总皂苷的吸附性能和洗脱参数。25mL辣木叶总皂苷样品液上大孔吸附树脂,用蒸馏水,30%、50%乙醇各200mL依次洗脱,辣木叶总皂苷富集于50%乙醇洗脱部位。采用大孔吸附树脂技术富集、纯化辣木叶总皂苷,其洗脱率为(76．3±0．3)%,精制度为124．9%,该法可较好地纯化辣木叶总皂苷。

To study the technological parameters of the purification process of saponins with macroporous adsorption resin. The adsorptive characteristics and elutive parameters of the process were studied by taking the elutive and purified ratio of saponins as markers. 25 mL of the extraction ofMoringa Oleifera was purified with a column of macroporous adsorption resin and washed with 200mL distilled water, then eluted with 200mL of 30% ethanol and 200mL of 50% ethanol. Most of saponins were collected in the 50% ethanol. With macroporous adsorption resin adsorbing and purifying, the elutive ratio of saponins is（ 76.3±0.3） % and the purity reaches 124.9 %.So this process of applying macroporous adsorption resin to adsorb and purify Saponins is feasible.