用PCR方法检测献血员的B_(19)病毒感染情况

Detection of Human parvovirus B 19 in Blood Donors with Polymerase Chain Reaction

目的：研究人微小病毒Ｂ１９在献血员中的感染情况。方法：采用ＰＣＲ技术对５００份献血员血清标本进行检测。结果：发现一例Ｂ１９病毒ＤＮＡ阳性，并用Ｋｏｃｈ设计的引物扩增标本和用ＨａｅⅢ，ＰｓｔⅠ和ＳｔｙⅠ酶切分析都证实为人微小病毒Ｂ１９。结论：国内献血者中有Ｂ１９病毒携带者。血液和血液制品有可能污染有Ｂ１９病毒。这个情况值得进一步研究。

bjective: Human parvovirus B 19 infection in blood donors has been researched. Methods: 500 serum samples of blood donors have been detected by using polymerase chain reaction. Results: Only one is B 19 DNA positive,the positive rate is 0.2%(1/500).It is proved that the positive is human parvovirus B 19 by amplification with other primers and digestion amplified fragments with HaeⅢ,Pst I and Sty I.Conclusion: There are B 19 carriers in blood donors in our country. BIood and blood products are possibly contaminated by human parvovirus B 19 .It is valuable that further research should be done on the case.