CD105、MMP-2和MMP-9在骨巨细胞瘤中的表达及意义

Expression and Significance of CD105,MMP-2 and MMP-9 in Giant Cell Tumor of Bone

目的探讨骨巨细胞瘤中CD105、MMP-2和MMP-9的表达及与肿瘤复发的关系。方法应用免疫组化SP法检测CD105、MMP-2和MMP-9在38例骨巨细胞瘤中的表达,分析它们与肿瘤临床病理参数及术后复发的关系。结果(1)CD105标记的肿瘤内MVD均值为9.70±2.34,浸润型肿瘤的MVD值明显高于静止型(P<0.05),复发组MVD值显著高于未复发组(P<0.01),而与组织学分级无关(P>0.05)。(2)MMP-2的阳性表达率为47.37%,其表达与复发有关(P<0.05),而与组织学分级、X线分型无关(P>0.05)。(3)MMP-9的阳性表达率为60.53%,与组织学分级无关(P>0.05),与X线分型和复发有关(P<0.01)。(4)MMP-2、MMP-9阳性组的MVD值分别高于相应的阴性组(P<0.01)。结论CD105标记的MVD值和MMP-2、MMP-9过表达可作为预测骨巨细胞瘤复发的客观依据。MMP-2、MMP-9过表达与肿瘤内新生血管的形成有关。

Objective To study the expression level of CD105,MMP-2 and MMP-9 in giant cell tumor of hone and to detect the correlation between their expression levels and tumor recurrence. Methods The expressions of CD105, MMP-2 and MMP-9 in 38 cases of giant cell tumor of hone were studied by using SP immunohistochemical method. Their relationship with clinicopathological features and tumor recurrence after operation was analysed too. Results （1） The average value of MVD in tumor was 9. 70 ± 2. 34, which was much higher in aggressive type cases than in quiescent type （P〈0. 05）. Recurrent cases had higher MVD value than cases without recurrence（P〈0. 01）, yet was not associated with histological grade（P〉0. 05）. （2） The expression ratio of MMP-2 was 47. 37%. Recurrent cases had higher positive rate than cases without recurrence （P〈 0. 05）. No distinct difference of the expression ratio was found among cases of different histological grade and radiological type （P〉0. 05）. （3） Positive rate of MMP-9 was 60. 53%. No distinct difference of the expression level of MMP-9 was found among cases of different histological grade（P〉0. 05）. Tumors of aggressive type and active type had higher positive rate than quiescent type cases（P〈0. 01 ） ,and recurrent cases had higher positive rate than cases without recurrence（P〈0. 01 ）. （4） MMP-2 positive cases had higher MVD than MMP- 2 negative cases （P〈0. 01 ）, MMP-9 positive cases had higher MVD than MMP-9 negative cases（P〈0. 01 ）. Conclusion MVD counted with CD105 and overexpression of MMP-2 and MMP-9 could be used as objective prognostic indicators of tumor recurrence. Angiogenesis in tumor might be promoted by the overexpression of MMP-2 and MMP-9.