抗生素生物合成基因簇克隆方法

A Brief Introduction to the Methods of Antibiotic Biosynthetic Gene Clusters Cloning

抗生素生物合成基因簇的克隆是药物创新和产量提高的必要前提,PCR技术、基因文库的构建及标记探针的筛选、异源宿主的表达和染色体步查等方法,常用于抗生素生物合成基因簇的克隆。载体系统及生物信息学的发展对基因克隆产生了重大影响。

The cloning of antibiotic biosynthetic gene clusters is essential for new drug discovery and yield improvement by metabolic engineering. The methods of polymerase chain reaction （PCR）, DNA library constructing and screening by labeling probe,expressing in heterogenesis host and genomic walking are often used for gene clusters cloning . The development of cloning vector and bioinformatics brings the technology of Gene Cloning to a new century.