猪小肠黏膜下层复合支架修复兔下颌骨缺损

Regeneration of rabbit mandibular defects with composite scaffold materials of SIS and nm β-TCP

目的应用猪小肠黏膜下层(SIS)与纳米β-磷酸三钙(β-TCP)结合的复合支架材料,修复兔下颌骨缺损。方法13只新西兰大白兔于双侧下颌骨制备骨缺损,分别应用SIS(SIS组)、纳米β-TCP(β-TCP组)以及两者混合制备成的复合支架材料(复合支架组)进行骨缺损的修复,并设置空白对照(对照组)。术后12周时缺损处取材,分别进行大体和组织学观察、X线检查及骨密度测定。结果术后12周时,新生骨小梁占据大部分缺损区域。复合支架组和SIS组的修复材料已基本降解,β-TCP组残余少量材料。复合支架组骨密度较高,新生骨小梁形态较成熟;对照组新生骨小梁较其他各组数量少且不规则,而胶原成分较多。结论猪SIS与纳米β-TCP复合支架材料,具有良好的成骨特性和组织相容性,能够有效地修复下颌骨缺损。

Objective To determine whether the small intestinal submucosa（SIS）/nano meter crystal β- tricalcium phosphate（ nm β- TCP） composite can enhance the regeneration of rabbit mandibular defects. Methods Twenty-six mandibular defects were made on thirteen New Zealand rabbits, and were ramdonly divided into four groups： group Ⅰ , single SIS was applied to each defects; group Ⅱ , nm β-TCP; groupⅢ , the composite scaffold materials of SIS and nm β-TCP; groupⅣ, control. Twelve weeks after the operation, the samples were extracted for gross observation, histological analysis, X-ray examination, and relative bone density （RBD） recording. Results Twenty weeks after the operation, the newborn bone trabecula took up most of the area with defects. The restorative materials in the SIS group and composite scaffold materials group had almost degraded, and little remained in the β-TCP group. The composite scaffold materials group was found more newborn bone trabecula with mature formation, and the average RBD was relatively higer, while less newborn bone trabecula with irregular formation and more collagen were observed in the control group. Conclusion The composite materials of SIS and nm β-TCP, which enjoy favourable bone formation characteristics and histocompatibility, can enhance bone regeneration in rabbit mandibular defects.