微胶囊固定的糖甙酶的失活机理研究

STUDY ON DEACTIVATION MECHANISM OF MICROENCAPSULATED βGLUCOSIDASE

在文献 [1 ]的基础上 ,进一步优化了微胶囊包埋糖甙酶的工艺条件。试验了三种成膜材料 :聚烯丙胺盐酸盐(PAAHC)、聚乙烯亚胺 (PEI)和聚乙二醇 (PEG)。实验结果表明 :PEG是一种较合适的包膜材料 ,用 PEG制备的微胶囊在反应开始时有较大的反应速度 ,降低缓冲液的浓度和交联剂的量制备的微胶囊可以有效地提高反应速度。用四种具有不同催化活性的微胶囊的重复使用试验表明 :重复使用的周期相差可达六倍 ,但总的产量大致相同。这一结果说明 。

The technology of microencapsulation of βglucosidase was optimized further on the base of literature: Three materials forming membrane, poly(allylamine hydrochloride), PAAHC; polyethyleneimine, PEI and poly(ethyleneglycol), PEG were tried. Experimental result showed that PEG was the best one. The microcapsules made using PEG had laeger reaction rate at beginning of reaction. The microcapsules prepared at lower buffer solution concentration and less crosslinking agent could effectively promote reaction rate. Reuse test using four types of microcapsules with different catalytic activity exhibited that the difference of reuse cycles could be as large as 6 times, but the total yields were almost the same. The result demonstrated that the main reason of deactivation of microencapsulated βglucosidase was not from the contact with organic solvent, but catalysis itself.