纤维素酶产生菌的筛选鉴定和产酶条件优化

Screening,Identification of Cellulase-production Strain and Optimization of Its Fermentation Conditions

筛选得到1株纤维素酶产生菌TC1,根据真菌的分类鉴定方法,鉴定为根霉属菌(Rhizopus sp.)。正交实验确定该菌株的优化培养条件为:稻草采用15%的醋酸预处理,稻草与麸皮比为3:7,固液比为1:3,接种量10%,温度28℃,稀释1倍的Mandels营养液作为培养基液体。在优化培养条件下培养96h,发酵终点时滤纸酶活FPA、CMC酶活分别达到25.5、249.0 U/g(干物质),较优化前的17.8、187.0 U/g(干物质)分别提高了43%和33%。

One cellulase-production strain TC1 was screened. It was identified as Rhizopus sp. based on identification method of fungi. The optimized fermentation conditions determined by orthogonal design were： straw powder（pretreated by 15% acetic acid）, straw powder/wheat bran（3 ： 7）, solid substrate/liquid nutriment （1 ： 3）, and quantity of inoculation（10%）, 28℃, and half time diluted Mandels was used as liquid nutriment. The maximum activities of filter paper enzyme（FPA） and carboxymethyl cellulose enzyme（CMC） were 25. 5,249. 0 U/g dry medium, respectively under the optimized fermentation conditions after 96h, which were increased by 43% and 33% , respectively compared with the initial one.