丙酸高产菌株的复合诱变选育

Compound Mutation Breeding of Propionic Acid High-yield Strain

以费氏丙酸菌IFFI.10019为出发菌株,经2次紫外线、2次亚硝基胍、1次亚硝基胍-氯化锂多重复合诱变处理,选育获得丙酸高产菌株NL—3,丙酸产量由原来的0.20g/L,提高到1.23g/L,提高率达到515%。实验证明采用多因子复合诱变,特别是NTG-LiCl复合诱变,能有效改变菌株对诱变因素的敏感性,提高变异率,逐步提高突变株的产丙酸水平。

Mutant（NL-3） Strain producting high-yield Propionic Acid was screened from Propionibacteria freudenreichii IFFI. 10019 after treated with UV two times, NTG for two times and NTG-LiCl one time. the Propionic Acid production level reached up to 1.23 g/L, with an increase rate of 515% compared to 0.20 g/L from original strain. The experiments showed that using compound mutation can alter the original stain＇s sensitivity to mutagenic agents, especially NTG-LiCl, increase mutation frequency and raise Propionic Acid yield.