吸附树脂分离大豆异黄酮染料木苷和乙酰基染料木苷的研究

Separate Soybean Isoflavone Genistin and Acetygenistin by Resin

利用Sepabeads SP207大孔吸附树脂分离提纯大豆异黄酮。用乙醇从脱脂大豆中提取的大豆异黄酮主要含有染料木苷,还含有大豆苷、黄豆苷和乙酰基染料木苷等;经Sepabeads SP207大孔吸附树脂的乙醇梯度洗脱,可分离得到染料木苷和乙酰基染料木苷;经HPLC检测其纯度均为90%以上。

The soybean isoflavone was extracted by alcohol and separated and purified by Sepabeads SP207 macro-pore resin. The extraction from soyabean by 70 % alchol mainlyy contained the genistin, daidzin, glycitin and acetygenistin. The pure genistin and acetygenistin were obtained by the Sepabeads SP207 macro-pore resin; the pure genistin and acetygenistin contents were over 90 % by the HPLC method.