槟榔碱对口腔黏膜肌成纤维细胞分化的影响

Effect of arecoline on the differentiation of myofibroblasts of oral mucosa

目的探讨口腔黏膜下纤维性变组织中肌成纤维细胞的来源。方法通过体外口腔黏膜角质形成细胞和成纤维细胞共培养,用免疫组化、反转录聚合酶链反应等方法观察α-平滑肌肌动蛋白的表达。结果体外培养的口腔黏膜下纤维性变成纤维细胞中α-平滑肌肌动蛋白的阳性率为(85.80±3.56)%,正常口腔黏膜成纤维细胞中阳性率为(3.82±0.76)%。槟榔碱直接干预成纤维细胞后α-平滑肌肌动蛋白的表达与对照组相比差异无统计学意义(P>0.05);角质形成细胞与成纤维细胞共同培养组成纤维细胞中α-平滑肌肌动蛋白的表达增强;角质形成细胞经槟榔碱预处理后与成纤维细胞共同培养组α-平滑肌肌动蛋白的表达强于无干预共同培养组。结论口腔黏膜下纤维性变组织中成纤维细胞向肌成纤维细胞分化可能是槟榔成分与角质形成细胞共同作用的结果。

Objective To investigate the origin of myofibroblasts in oral submucous fibrosis. Methods The oral keratinocytes and fibroblasts were isolated and cultured. The expression of the α-smooth muscle actin in the fibroblasts was examined by immunohistochemistry and reverse transcriptase polymerase chain reaction（RT-PCR）. Results No difference was found in expression of α-smooth muscle actin between the fibroblasts that were directly stimulated by arecoline and the control. The expression of α-smooth muscle actin in the keratinocyte and fibroblast-cocultured group was higher than in the control group, and higher in fibroblasts cocultured with keratinocytes preprocessed by arecoline than in fibroblasts cocultured with keratinocytes without preprocessed by arecoline. Conclusions The differentiation of myofibroblasts from fibroblasts in oral submucous fibrosis might be induced by the interaction of arecoline and keratinocyte.