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秀丽线虫sod-3基因RNA干扰及早衰模型的建立 被引量：1

Model of Rapid Aging Based on RNAi-Mediated Gene Silencing of sod-3 Expression in Caenorhabditis Elegans
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摘要 利用RNA i技术,通过干扰秀丽线虫中的sod-3基因表达,建立了因超氧化物歧化酶3(SOD3)表达缺失引起快速衰老的线虫模型,为sod-3基因功能的研究以及相关疾病的基因治疗等提供动物模型. According to the oxidative damage theory of aging, free radicals, particularly reactive oxygen species （ROS）, are responsible for the senescence and many human diseases. ROS are generated during normal metabolic process in all the oxygen-utilizing organisms. They are detoxified by superoxide dismutase. The manganese-containing superoxide dimutase 3 （SOD3） has been shown to play a role in the aging process in Caenorhabditis elegans. In this research, we show that sod-3 RNA interference （RNAi） induces insenecence in Caenorhabditis elegans as expected. This method can be used to understand the process of aging and the mechanism of antioxidance.
作者 关树文 黄磊 罗晶 韩秋菊 王丽萍 李惟
机构地区 吉林大学生命科学学院 东北师范大学遗传与细胞研究所
出处 《吉林大学学报（理学版）》 CAS CSCD 北大核心 2007年第4期676-680,共5页 Journal of Jilin University:Science Edition
基金 长春市振兴老工业基地科技攻关项目及长春市与吉林大学生命科学学院合作计划项目基金(批准号:04-02GG221)
关键词 RNAI 秀丽线虫 超氧化物歧化酶3 RNAi Caenorhabditis elegans superoxide dismutase 3
分类号 Q789 [生物学—分子生物学]
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参考文献16

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同被引文献14

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吉林大学学报（理学版）
2007年 第4期

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