摘要
利用RNA i技术,通过干扰秀丽线虫中的sod-3基因表达,建立了因超氧化物歧化酶3(SOD3)表达缺失引起快速衰老的线虫模型,为sod-3基因功能的研究以及相关疾病的基因治疗等提供动物模型.
According to the oxidative damage theory of aging, free radicals, particularly reactive oxygen species (ROS), are responsible for the senescence and many human diseases. ROS are generated during normal metabolic process in all the oxygen-utilizing organisms. They are detoxified by superoxide dismutase. The manganese-containing superoxide dimutase 3 (SOD3) has been shown to play a role in the aging process in Caenorhabditis elegans. In this research, we show that sod-3 RNA interference (RNAi) induces insenecence in Caenorhabditis elegans as expected. This method can be used to understand the process of aging and the mechanism of antioxidance.
出处
《吉林大学学报(理学版)》
CAS
CSCD
北大核心
2007年第4期676-680,共5页
Journal of Jilin University:Science Edition
基金
长春市振兴老工业基地科技攻关项目及长春市与吉林大学生命科学学院合作计划项目基金(批准号:04-02GG221)