摘要
目的通过检测9个微卫星序列多态性位点以鉴别完全性葡萄胎的亲代来源。方法用多重 PCR 技术和变性聚丙烯酰胺凝胶电泳结合银染显色方法,对50例病理诊断为完全性葡萄胎患者的葡萄胎标本及夫妇外周血标本进行 DNA 分析。结果 50例完全性葡萄胎患者中,双亲来源完全性葡萄胎7例,占14%;孤雄完全性葡萄胎43例,占86%,其中孤雄单精完全性葡萄胎35例,孤雄双精完全性葡萄胎8例。结论微卫星序列多态性位点检测是一种鉴别完全性葡萄胎亲代来源的简便、快速、可靠、高效的方法。
Objective Through detection of 9 loci of polymorphisms of microsatellite to investigate the parental origin of complete hydatidiform mole. Methods Using the technology and method of multipolymerase chain reaction and electrophoresis in denatured polyacrylamide gel and sliver stain detection, we carried out DNA analysis on 50 cases of complete hydatidiform mole diagnosed by histopathology and 50 copies of the couples' peripheral blood. Results There are 7 cases of biparental complete hydatidiform mole ( 14% ) ; and 45 cases of androgenetic complete hydatidiform mole ( 86% ) among 50 cases of complete hydatidiform mole. Conclusions Detection of polymorphisms of microsatellite is a technology that can be used to identify the parental origin of complete hydatidiform mole. It is simple, quick, reliable and highly efficient.
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
2007年第7期468-471,共4页
Chinese Journal of Obstetrics and Gynecology
基金
浙江省医药卫生科学研究基金(2005A061)
关键词
葡萄胎
串联重复序列
多态性
限制性片段长度
Hydatidiform mole
Tandem repeat sequences
Polymorphism,restriction fragment Iength