体外诱导人类胚胎干细胞定向分化为神经干细胞

Differentiation of human embryonic stem cells into neural stem cells in vitro

目的探索一种诱导人类胚胎干细胞(hESCs)向神经干细胞(NSCs)定向分化的简单高效的方法。方法将 hESCs 在细菌培养皿中以无血清培养基悬浮培养形成拟胚体,进一步将成熟拟胚体打散、贴壁培养于含有 B27和 noggin 等成分的特定培养基中,诱导其向神经干细胞定向分化。结果悬浮于细菌培养皿中的 hESCs 不贴壁,进行性长大,7～10 d 形成成熟拟胚体;拟胚体在特定培养基中可诱导成高纯度(约96.4%)的 nestin 阳性细胞(即 NSCs),进一步培养,这些细胞可分化成各种成熟的神经细胞。结论该方法诱导 hESCs 向 NSCs 定向分化较文献报道耗时更短、费用低廉且效率更高,为进行细胞移植治疗神经系统相关疾病提供了很好的种子细胞来源。

Objective To explore a kind of simple and high efficient approach to differentiate human embryonic stem ceils （hESCs） into neural stem ceils（NSCs）. Methods hESCs were cultured in bacterial culture dish filled with serum free medium to gain embryoid bodies. Then the mature embryoid bodies grew in the special medium including B27 and noggin by adherent culture to differentiate into NSCs. Results The hESCs kept floating in the bacterial culture dish and growing all the time and then formed mature embryoid bodies 7 to 10 days later. The embryoid bodies could be differentiated into highly pure （96.4%） nestin positive cells. And these ceils were differentiated into all kinds of neural cells if cultured further. Conclusions This kind of method is less time-consuming, cheaper, and more efficient than those of the results in literatures reported. It affords very good source of seed cells for ceil transplantation therapy in the future.