10种常见SPF级实验大、小鼠血液学及生化指标正常参考值的探讨

A Probe into Normal Levels of Hematological and Biochemical Indexes in 10 kinds of Common SPF Rats and Mice

目的建立10种常见SPF级实验大、小鼠血液及生化正常参考值。方法采用半自动生化测定仪及血球计数仪对不同周龄、不同性别的常见10种SPF大鼠的血液学指标及生化指标值进行测定。结果取得4周龄和8周龄不同周龄、不同性别大、小鼠血液学及生化值平均值及正常参考范围;多数生化指标受年龄及性别的影响,除了不同品系、年龄和性别的大、小鼠ALP呈现规律性变化,其他指标也与相关报道有差异。结论在基础生物学研究中,要充分考虑到性别和年龄对实验动物血液学及血清生化指标的影响。

Objective To obtain the reference value of normal hematological and biochemical indexes of 10 kinds of usual SPF grade rats and mice. Methods The some hematological and biochemical indexes were determined by semiautomatic biochemical analyzer and hemacytometer in 10 kinds of usual SPF grade rats and mice of different ages and sexes. Results The some hematological and biochemical indexes of 10 kinds of usual SPF male and female rats and mice at four and eight weeks of age were obtained. Rather striking age-related and sex-related differences have been observed concerning some biochemical indexes in those rats and mice. The regular change of alkaline phosphatase was found in the rats and mice of different strains, ages, and sexes. Conclusion There are rather striking age-related, sex- related, and strain-related differences in some hematological and biochemical indexes of usual SPF grade rats and mice, which should be considered in the basal biological researches.