摘要
目的建立猪细小病毒血清学检测方法。方法用抗猪细小病毒的猪源性血清,通过IFA和IEA两种方法,首先将阳性血清吸附PK-15细胞抗原,以排除非特异反应,通过对阳性血清及3种二抗(其中IFA以FITC标记的抗猪IgG抗体,IEA分别以HRP标记的抗猪IgG抗体和SPA-HRP作为二抗)进行浓度梯度滴定,确定最佳工作浓度,并比较三种反应体系的敏感性。结果IFA最高能检测出PPV-猪组织混合感染PK-15细胞模型中1个TCID50,而其它两种反应体系均只能检测100个TCID50。结论间接免疫荧光检测方法比其它两种方法敏感性更高。
Objective To establish the serological method for detection of porcine parvovirus （PPV）. Methods Firstly, the swine serum containing the antibodies against PPV was absorbed by the PK-15 to eliminate nonspecific reaction, next, the optimal reaction was determined by concentration gradient titration of the serum and the second reaction-antibodies, and then, the sensitivity among the reaction systems was compared. Results 1 TCID50 could be acquired with IFA, however, only 100 TCID50 could be acquired with the other two methods. Conclusion IFA method was more sensitive than the other two methods.
出处
《实验动物科学》
2007年第2期56-59,共4页
Laboratory Animal Science
基金
863计划:生物技术产品的质量标准和检测技术平台研究。2003AA2Z3481
