胃癌及胃炎的临床诊断中红外光谱方法研究

Clinical Study on Gastric Carcinoma and Gastritis Using mid-FT-IR Spectroscopy

用中红外光谱ATR方法对110例冻存,89例新鲜离体胃组织样品,80例新鲜胃镜样品进行研究,同时用中红外光纤法进行了6例在体原位的检测。对110例冻存样品,各项指标与病理检测结果的符合率平均可达到90%以上。新鲜离体与在体原位也大致同病理结果符合。为了研究地区差别,对杭州市第一医院消化科80例胃镜样品进行了光谱测定,根据病变程度可以看到相应的光谱变化。结果表明中红外光谱法用于各种胃炎等胃部疾病和恶性肿瘤的检测是一种有效方便的方法。

110 gastric disease samples stored in liquid nitrogen, 89 fresh gastric disease samples after operation and 80 fresh tissues obtained from gastroscope were investigated using mid-FTIR ATR method. At the same time, the spectra of gastric tumor of six patients were measured in vivo in the process of operation using mid-IR fiber optics technique. The detected indexes from IR were compared with the pathological biopsy and the results show that various indexes are in agreement with the results of pathological diagnosis more than 90% . The same consequences were observed for fresh tissues and samples in vivo detection. To investigate the difference of the incidence of this disease in south and north of China, 80 fresh tissues from gastroscope, including various types of gastric diseases, for example, superficial gastritis, chronic gastritis, moderate atrophic gastritis and severe atrophic gastritis, and so on, obtained from Hangzhou First People＇s Hospital were measured. The spectroscopic measurements show that different stages of pathologic changes of stomach have corresponding spectroscopic characteristics. The results demonstrate that mid-IR ATR method is an effective Way for detection of various gastritis diseases and carcinoma of stomach.