厚叶悬蒴苣苔BcWRKY1转录因子基因的克隆及初步的功能分析

Molecular Cloning of BcWRKY1 Transcriptional Factor Gene from Boea crassifolia Hemsl and Its Preliminary Functional Analysis

通过RT-PCR程序,从经过SA诱导的厚叶悬蒴苣苔中获得含WRKY家族保守序列的一条cDNA片段。运用RACE(Rapid Amplification of cDNA Ends)技术获得全长1803bp的cDNA克隆,名之为BcWRKY1。序列分析表明:BcWRKY1与甘薯SPF1[D30038]相似性最高,保守区同源性达到84%。初步的Northern杂交分析表明:干旱、低温、高盐等逆境胁迫和外加SA、MeJA、JA、ABA等信号分子的诱导均能提高BcWRKY1基因的表达。但是表达情况各不相同。150mmol/L NaCl对BcWRKY1的诱导作用尤为明显和迅速。2168bp的BcWRKY1的基因组DNA克隆亦已获得,序列分析表明它含有4个内含子。

A piece of cDNA fragment by RT-PCR from the Boea crassifolia Hemsl induced by SA were obtained. This fragment contains a conservative WRKY-domain sequence. The full-long cDNA sequence about 1 803 bp were cloned by the RACE（Rapid Amplification of cDNA Ends） and named BcWRKY1. The results of sequence analysis indicate that BcWRKY1 is most similar to Ipomoea batatas SPF1 [ D30038], the conservative sequence alignment score is 84%, The Northern blot analysis show that BcWRKY1 gene can be induced by the abiotic stresses like drought, soil salinity, low temperature and the molecular signal such as SA, MeJA, JA, ABA, but all the expression behaviors of them are different. The BcWRKY1 gene is rapidly and strongly induced by 150 mmol/L NaCl. The BcWRKY1 genomic DNA about 2168bp nucleotides has been cloned and the results of sequence analysis indicate it contains four introns.