摘要
根据南芥菜花叶病毒(Arabis mosaic Nepovirus)CP基因序列,设计并合成了特异性RT-PCR检测引物,对南芥菜花叶病毒和非南芥菜花叶病毒的感病植物组织进行了PCR扩增反应。结果,南芥菜花叶病毒的感病植物组织的PCR产物均出现364bp的特异性扩增条带,而非南芥菜花叶病毒均未出现扩增条带,证明这对引物具有南芥菜花叶病毒鉴定特异性。将提取的南芥菜花叶病毒总RNA做梯度稀释,测定该检测体系的敏感度,结果表明,此体系最低可检出南芥菜花叶病毒提取的RNA模板浓度为150pg/μL。
Specific primers which were designed based on the sequence of CP gene in ArMV by RT - PCR were used for PCR reaction of plant tissue with ArMV and non - ArMV, only the plant tissue with ArMV had the specific amplification of a 364 bp DNA product. The primer was specific to ArMV. The RNA solution of ArMV was diluted by grads to determined sensitivity of the system. The level of detection limit of the primer was 150pg/μL.
出处
《江西农业学报》
CAS
2007年第7期34-35,共2页
Acta Agriculturae Jiangxi
基金
国家质检总局植物病原检测专项基金(2005IK066)
