两种大肠癌细胞株小鼠肝转移模型的建立及比较

Establishment and comparison of liver metastasis models of two colorectal carcinoma cell lines in mice

目的建立人、鼠大肠癌细胞肝转移动物模型,为研究大肠癌肝转移机制及筛选、评估抗转移药物提供工具。方法选取鼠大肠癌细胞株CT26和人大肠癌细胞株LoVo,分别接种于Balb/c小鼠及裸鼠脾脏内,观察其成瘤情况、肝脏转移率及生存期,并对两种模型进行初步比较。结果两种细胞株均能复制出大肠癌肝转移动物模型,病理结果证实肝转移肿瘤符合典型的低分化腺癌特征。CT26细胞在免疫功能完整的小鼠体内,仍具有良好的成瘤性和转移率,较LoVo细胞建立的裸鼠肝转移模型转移率更高,成瘤时间更短。结论成功复制出两种人鼠大肠癌细胞株肝转移动物模型,均能较好模拟大肠癌体内肝转移过程。

Objective To establish hepatic metastasis models of two colorectal carcinoma cell lines in mice for studing mechanism involved in colorectal carcinoma metastasis and its potential countermeasures. Methods Murine and human colorectal carcinoma CT26 and LoVo cells were inoculated into the spleen of Balb/c mice and Balb/c nude mice, respectively. The conditions of all the mice were observed, and the survival time and liver metastases were recorded. Results All mice inoculated with CT26 cells and a few with LoVo cells developed liver metastases without metastases in any other organs. Pathological examination identified the liver metastatic foci as poorly differentiated colonic adenocarcinoma. Compared with the mice inoculated with LoVo cells, those with CT26 cells had a higher rate of liver metastasis and a shorter survival time. Conclusion The mouse model has been established successfully, which well mimics the pathological process of liver metastasis of colorectal cancer.