体外受精-胚胎移植周期颗粒细胞解偶联蛋白2的表达与胚胎发育的关系

Uncoupling protein-2 expression in granular cells and embryo development in in vitro fertilization-embryo transfer cycle

目的探讨体外受精周期颗粒细胞解偶联蛋白2(UCP2)的表达与细胞内活性氧水平关系以及对胚胎发育的影响。方法运用UCP2抗体和DCFH-DA,采用流式细胞仪技术,分别检测了53例体外受精周期颗粒细胞UCP2表达和细胞内活性氧水平。其中≤35岁组35例,>36岁组18例。结果颗粒细胞UCP2的表达与细胞内活性氧水平负相关(r=-0.578,P<0.01),并且,年长妇女颗粒细胞UCP2表达下降,UCP2表达低者,良好胚胎形成率降低,取卵数大于等于15个的患者颗粒细胞UCP2表达升高,卵母细胞成熟比例低。结论颗粒细胞通过增加UCP2表达反馈性抑制细胞内活性氧水平过高,进而控制卵泡液活性氧水平,保护卵母细胞免受氧化应激损伤。

Objective To examine the expression of uncoupling protein-2 （UCP2） in the granular cell of women undergoing in vitro fertilization （IVF） and explore its role in embryo development. Methods In this prospective study, the levels of UCP2 and reactive oxygen species （ROS） were measured by the chemiluminescence method in the granular cell of 53 women. Results Women with low UCP2 level had higher ROS level, suggesting an inverse relationship between them （r=-0.578, P〈 0.01）, and their oocyte development was impaired. Granular cells of elder women exhibited lowered UCP2 expression. Conclusion The granular cells increase UCP2 expression to suppress the elevation of intracellular ROS level through a feedback mechanism and therefore protect the oocytes against oxidative stress.