摘要
瞄准：在鼠科的附睾头在类脂化合物作文和基因表示规定调查肝 X 受体(LXR ) 的角色。LXR 是为 oxysterols 的原子受体，分子源于作为二 isoforms 在哺乳动物是在场的胆固醇新陈代谢：LXR α，是明确地在类脂化合物使物质替换纸巾表示的更多，脂肪质并且 steroidogenic 纸巾，和巨噬细胞，而 LXR β是无所不在的。在繁殖生理学的 Theirimportance 被两 LXR 的功能在被破坏了的雄的老鼠有富饶骚乱在 5 个月岁时开始的事实支撑了，导致到 9 个月的年龄的完全的绝育。这些缺点在头片断与 epididymalepithelial 退化被联系一和二，并且与一个精子中间的片脆弱，当钠满足时，导致孤立的精子头和毛虫的存在从附睾尾被恢复。方法：野类型的 andLXR 缺乏的老鼠的附睾头的类脂化合物作文用油红Ｏ在织物 cryosections 上染色被估计，类脂化合物抽取由高效液相色谱法或煤气的层析列在后面。基因表示被量的实时聚合酶链反应检查。结果：用 LXR 缺乏的鼠标，我们显示出附睾头的类脂化合物作文以及编码 SREBPlc， SCD1 和 SCD2 的基因的一个显著地减少的表达式的改变，在脂肪酸新陈代谢包含了。结论：总的来说， LXR 是重要管理者 ofepididymal 的这些结果表演工作，并且在成熟处理发生在精子附睾成熟期间的类脂化合物起一个关键作用。
Aim： To investigate the roles of liver X receptors （LXR） in the lipid composition and gene expression regulation in the murine caput epididymidis. LXR are nuclear receptors for oxysterols, molecules derived from cholesterol metabolism that are present in mammals as two isoforms： LXRα, which is more specifically expressed in lipid-metabolising tissues, such as liver, adipose and steroidogenic tissues, and macrophages, whereas LXRβ is ubiquitous. Their importance in reproductive physiology has been sustained by the fact that male mice in which the function of both LXR has been disrupted have fertility disturbances starting at the age of 5 months, leading to complete sterility by the age of 9 months. These defects are associated with epididymal epithelial degeneration in caput segments one and two, and with a sperm midpiece fragility, leading to the presence of isolated sperm heads and flagella when luminal contents are recovered from the cauda epididymidis. Methods： The lipid composition of the caput epididymidis of wild-type and LXR-deficient mice was assessed using oil red O staining on tissue cryosections and lipid extraction followed by high performance liquid chromatography or gas chromatography. Gene expression was checked by quantitative real time polymerase chain reaction. Results： Using LXR-deficient mice, we showed an alteration of the lipid composition of the caput epididymidis as well as a significantly decreased expression of the genes encoding SREBPlc, SCD1 and SCD2, involved in fatty acid metabolism. Conclusion： Altogether, these results show that LXR are important regulators of epididymal function, and play a critical role in the lipid maturation processes occurring during sperm epididymal maturation. （Asian J Androl 2007 July; 9： 574-582）
