Protamex蛋白酶水解大豆分离蛋白的研究

Hydrolysis of soy protein isolate by Protamex protease

以大豆分离蛋白为底物,通过响应面分析,确定了用Protamex蛋白酶水解的最佳条件。最佳酶解条件为:大豆分离蛋白浓度6.0%,加酶量2.4%,酶解温度51℃,酶解时间4h,pH7.0。在最佳条件下,可得到水解度为12.94%的酶解液。

Using soy protein isolate as the substrate,the best conditions of enzymatic hydrolysis with Protamex protease were obtained by response surface method. The optimum hydrolyzing conditions were as follows： proportion of Protamex protease to substrate 2.4%,substrate concentration 6.0%,temperature 51 ℃,pH7.0 and hydrolysis time 4h. Under the best conditions,the degree of hydrolysis could reach 12.94%.