5种着色氧化锆陶瓷的细胞毒性评价

Evaluation of the cytotoxicity of five kinds of colorized zirconia

目的:对掺杂5种微量着色剂的氧化锆陶瓷的生物安全性进行初步评价。方法:采用四氮唑盐比色法(MTT)细胞毒性评价方法,用5种着色氧化锆陶瓷材料的浸提液体外培养L929小鼠成纤维细胞2d、4d、7d,于倒置相差显微镜下观察细胞形态;用MTT检测各实验组和对照组的吸光度值(OD值),计算各组细胞的相对增殖率,并按照6级毒性分类法对各实验组进行评级。结果:培养期细胞贴壁生长,细胞形态正常。随着培养天数增加细胞大量增殖,各实验组的毒性评级为0-1级。结论:添加5种微量着色剂的氧化锆陶瓷无细胞毒性,具备体内应用的生物安全基础。

Objective： The biological safety of zirconia ceramics dopanted with 5 kinds of colorants were tested by in vitro cytotoxicity. Method： The fibroblasts of L929 mouse were cultured in the extract of different materials for 2,4, 7 days respectively. The morphology of the cells were observed by microscope,and the proliferation of cells were examined using tetrazolium colorimetric assay. Result： During all culture periods, L929 cells showed normal morphology and proliferation in- creased with culture time.The toxicity gradation of all groups was 0-1.Conclusion：This study initially demonstrated that the five kinds of colorized zirconia had excellent biological safety.