摘要
目的:对掺杂5种微量着色剂的氧化锆陶瓷的生物安全性进行初步评价。方法:采用四氮唑盐比色法(MTT)细胞毒性评价方法,用5种着色氧化锆陶瓷材料的浸提液体外培养L929小鼠成纤维细胞2d、4d、7d,于倒置相差显微镜下观察细胞形态;用MTT检测各实验组和对照组的吸光度值(OD值),计算各组细胞的相对增殖率,并按照6级毒性分类法对各实验组进行评级。结果:培养期细胞贴壁生长,细胞形态正常。随着培养天数增加细胞大量增殖,各实验组的毒性评级为0-1级。结论:添加5种微量着色剂的氧化锆陶瓷无细胞毒性,具备体内应用的生物安全基础。
Objective: The biological safety of zirconia ceramics dopanted with 5 kinds of colorants were tested by in vitro cytotoxicity. Method: The fibroblasts of L929 mouse were cultured in the extract of different materials for 2,4, 7 days respectively. The morphology of the cells were observed by microscope,and the proliferation of cells were examined using tetrazolium colorimetric assay. Result: During all culture periods, L929 cells showed normal morphology and proliferation in- creased with culture time.The toxicity gradation of all groups was 0-1.Conclusion:This study initially demonstrated that the five kinds of colorized zirconia had excellent biological safety.
出处
《临床口腔医学杂志》
2007年第7期405-408,共4页
Journal of Clinical Stomatology
基金
上海市科委基金资助项目(03JC14053)
上海市重点学科资助项目(T0202)
关键词
氧化锆陶瓷
细胞毒性
四氮唑盐比色法
着色剂
zirconia ceramic
cytotoxicity
tetrazolium-based colorimetric assay: colorant