麻醉药物对细胞因子白细胞介素-1β和白细胞介素-6的影响

Effects of different anesthetics on IL-1β and IL-6

目的比较不同麻醉药物对白细胞介素(IL)-1β和IL-6的影响。方法选取健康自愿者6名,取其外周血以分层液密度梯度离心法分离出外周血单核细胞(PBMCs)。以内毒素(LPS)1μg/ml(终浓度)刺激,分为8组:①空白组(PBMCs+磷酸盐缓冲液);②损伤组(PBMCs+LPS 1μg/ml);③异丙酚组(PBMCs+LPS 1μg/ml+异丙酚4μg/ml);④氯胺酮组(PBMCs+LPS 1μg/ml+氯胺酮250μg/ml);⑤芬太尼组(PBMCs+LPS 1μg/ml+芬太尼5ng/ml);⑥咪唑安定组(PBMCs+LPS 1μg/ml+咪唑安定500ng/ml);⑦利多卡因组(PBMCs+LPS 1μg/ml+利多卡因2μg/ml);⑧布比卡因组(PBMCs+LPS 1μg/ml+布比卡因500 ng/ml)。经孵育离心后,采用酶联免疫吸附试验(ELISA)测定IL-1β和IL-6的生成。结果临床治疗浓度的异丙酚和氯胺酮对LPS刺激的PBMCs表达IL-6均有明显抑制作用,氯胺酮还能明显抑制IL-1β的表达。低浓度芬太尼、咪唑安定、利多卡因、布比卡因对IL-1β和IL-6的表达无明显影响。结论静脉麻醉药异丙酚和氯胺酮可抑制LPS刺激的PBMCs细胞因子的表达。

Objective To evaluate the effects of different anesthetics on IL-1 β and IL-6. Methods Peripheral blood mononuclear cells （PBMCs） were separated from the whole blood samples obtained from 6 healthy subjects according to Ficoil＇s method and then divided into 8 groups. The PBMSs in group 1 were added with phosphate buffer solution, stimulated with 1 μg/ml lipopolysaccharide （LPS） in group 2, and added with different anesthetics （propofol 4 μg/ml, ketamine 250 μg/ml, fentanyl 5 ng/ml, midazolam 500 ng/ml, lidocaine 2 μg/ml, bupivacaine 500 ng/ml, respectively） in groups 3 to 8, which were also LPS-stimulated. Productions of IL-1β and IL-6 were measured using ELISA. Results At therapeutic ranges, propofol and ketamine exhibited significant inhibition of IL-6 production by LPS-stimulated PBMCs, and ketamine also inhibited the expression of IL-1β. No such effects were seen with low level of fentanyl, midazolam, lidocaine and bupivacaine on the expression of IL-1β and IL-6. Conclusion Propofol and ketamine may inhibit the enhanced expression of cytokines produced by LPS-stimulated PBMCs.