摘要
为了筛选出耐热荷斯坦奶牛的分子遗传标记,并应用这些分子标记进行高产耐热荷斯坦奶牛的选育。从高产奶牛群中筛选出耐热性状差异显著的10头高产荷斯坦牛作为实验材料,采用RAPD作为分子标记筛选方法,优化奶牛RAPD反应最佳体系,并在154条随机引物中筛选出稳定性好、带型清晰的引物64条。通过RAPD结果,并结合红细胞钾测定结果和生产性能进行分析,确定3个分子标记与奶牛耐热性状相关,并对其进行了测序与分析。
In the paper, the research purpose is selecting heat-tolerance molecular markers from Holstein cattle and applying it to the research that helps to establish high-yield and high heat tolerance dairy cattle breed. With 10 high yield and different heat-tolerance Holstein Cattle as materials, we was optimized RAPD reaction system, and 64 primers with better result were selected in 154 random primers. Considered the detected result of potassium content in erythrocyte and production performance, three molecular markers relative to heattolerance in Holstein Cattle were selected and sequenced.
出处
《中国奶牛》
2007年第7期9-12,共4页
China Dairy Cattle
基金
湖南省重大简单科技专项(编号:05NK2004)
关键词
荷斯坦奶牛
RAPD
耐热
分子标记
Holstein Cattle
RAPD
Heat-tolerance
Molecular marker
