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鸭肝炎病毒基因组3′末端序列的克隆和分析 被引量：73

Molecular Cloning and Analysis of the 3′ Terminal Sequence of Duck Hepatitis Virus Genome
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摘要 用3′RACE和RT-PCR扩增并克隆鸭肝炎病毒（Duck hepatitis virus,DHV）Ⅰ型毒株C80和Ⅰ型变异株E63的3′末端序列。分析结果显示,C80株和E63株基因组3′末端均包含1 359 nt的3D、终止密码子TGA、长314nt的3′UTR,而poly（A）尾分别含18个A和19个A。由2株DHV 3D核苷酸序列所推导的3D蛋白均含453个氨基酸,均包含KDELR、DxxxxD、GxxCSGxxxTxxxNS、YGDD和FLKR等小RNA病毒RNA聚合酶的特征基序,该结果进一步证实Ⅰ型DHV属于小RNA病毒科的成员。两株DHV与小RNA病毒科9个已知属之间3D蛋白的氨基酸序列同源性为16%-37%,介于属间3D蛋白的氨基酸序列同源性范围（18%-60%）之内;此外,Ⅰ型DHV的3′UTR在小RNA病毒科是最长的。用3D蛋白进行进化分析的结果表明,Ⅰ型DHV可能属于小RNA病毒科的一个独立的病毒属。 The authentic 3＇ terminal sequences of genomes of duck hepatitis virus （DHV） serotype Ⅰ strain C80 and serotype Ⅰ variant strain E63 were obtained by using 3＇RACE and RT-PCR techniques. The analysis showed that 3＇terminal sequences in the genomes of the two DHV strains include the 3D region of 1359 nucleotides （nt）, the terminator codon TGA, and 3＇untranslated region （UTR） of 314 nt. The poly （A） tails of C80 and E63 are 18 nt and 19 nt in length respectively. The deduced 3D proteins of 453 amino acids of both DHV strains contain the motifs KDELR, DxxxxD, GxxCSGxxxTxxxNS, YGDD, and FLKR characteristic for RNA polymerase of picornaviruses, which confirms DHV serotype Ⅰ to be a member of Picornaviridae . The amino acid sequence identities among 3D protein of the two DHV strains with representatives of nine other picornavirus genera range from 16% to 37%, which are within the value ranges （18%-60%） of 3D amino acid sequence identities obtained from inter-genera comparisons. In addition, DHV serotype Ⅰ possesses the longest 3＇UTR in the family Picornaviridae . Phylogenetic analysis of 3D proteins indicated that DHV serotype Ⅰ may belong to a separated genus of the family Picornaviridae.
作者 丁春宇 张大丙
机构地区 中国农业大学动物医学院农业部预防兽医学重点开放实验室
出处 《病毒学报》 CAS CSCD 北大核心 2007年第4期312-319,共8页 Chinese Journal of Virology
关键词 鸭肝炎病毒 3D基因 3′UTR POLY(A) 3′RACE duck hepatitis virus 3D gene 3＇UTR poly （A） 3＇RACE
分类号 S858.32 [农业科学—临床兽医学]
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参考文献18

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病毒学报
2007年 第4期

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