肝细胞粘弹特性及其与细胞骨架结构的相关性研究

Investigation on the Viscoelastic Properties of Hepatocytes and Their Relavances to Cytoskeleton Structure

用微管吸吮技术研究了正常原代胎肝细胞（HFH）和肝实质细胞癌（HCC）细胞的粘弹特性。以此为基础，进一步研究细胞松驰素D（CD）和秋水仙素（Col）等两种细胞骨架干扰剂对HFH粘弹特性的影响。结果表明：HCC细胞的弹性系数K1、K2明显高于HFH的相应值，细胞松驰素D的作用引起HFH的弹性系数K1、K2和粘性系数。值均明显降低，秋水仙素的作用引起HFH的K1值增高，K2和μ值明显降低或趋于降低。作者对这一结果进行了简要讨论。

Using micropipette aspiration technique, the authors investigated the viscoelastic properties of humanfetal hepatocytes (HFH) and hepatocellular carcinoma (HCC) cells. And further. the effects of two cytoskeletoninterferents, cytochalasin D and colchicine on the viscoelastic properties of HFHs were investigated. The resultsshowed: the elastic coefficients K, and K, of HCC cells were significantly higher than those of HFHs; the actionof cytochalasin D led to an obvious decrease in elastic coefficients K1, K2 as well as the viscous coefficient μ ofHFHs ; under the action of colchicine, the K1 value of HFHs increased while the K2 and values decreased obviouslyor tended to decrease. A brief discussion was presented about these results.