L-天冬氨酸生物反应器的最优组合

THE OPTIMAL COMBINATION OF ENZYME REACTOR FOR L-ASPARTIC ACID

采用本文作者提出的酶反应动力学方程，比较分析了CSTR、PFR及CSTR串联PFR反应器生产L-天冬氨酸的能力。结果表明，对于以单位重量大肠杆菌细胞为基准的L-天冬氨酸的生产能力，当底物富马酸的转化率低于80％时，CSTR的生产能力最大；而当转化率高于94％时，PFR的生产能力高于CSTR的生产能力．在转化率高于80％的条件下，CSTR串联PFR的生产能力高于单独使用CSTR或单独使用PFR其最优组合方式关系到生产过程所要求的底物转化率．在最终转化率为99％的情况下，CSTRPFR及最佳CSTR＋PFR的生产能力分别为2.05，18．54，26．33[mol（L-天冬氨酸）／kg（湿细胞）·hr]．

The productivity of L-aspartic acid in CSTR, PFR and CSTR+PFR were comparably analysed using the equation of enzyme reaction kinetics developed by author.The results shown that the productivity of CSTR is the greatest on the base of eschrischia coli celIs per gram when the conversion of L-aspartic acid from fumaric aci is less than 80%.When the conversion is greater than 94%, the productivity of PFR is higher than that of CSTR. The productivity of CSTR+PFR is greater than that of individual CSTR or PFR under the condition that the converion greater than 80%. The optimal combination of CSTR and PFR is a function of conversion demanded the production of L-aspartic acid. At 99%of conversion, the productivity of CSTR, PFR and optimal combination of CSTR+PFR are 2.05, 18.54 and 26.33 [mol (L-aspartic acid)/kg (cells). hr], respectively.