HPLC法测定人血浆中麦考酚酸的浓度

Determination of mycophenolic acid in human plasma by HPLC

目的 建立人血浆中麦考酚酸酯活性代谢物麦考酚酸的HPLC测定法.方法 色谱柱为HYPERSIL C18（150 mm×5.0 mm，5 μm），预柱为岛津ODS（5.0 mm×3.9 mm，10 μm）.流动相为甲醇-磷酸二氢钠缓冲液（pH=3.25）-乙腈（40：40：20，V/V），流速0.8 mL·min^-1，检测波长254 nm，柱温25℃.结果 麦考酚酸的血浆样品为0.23～46.13 mg·L^-1时线性良好（r=0.999 7）.方法 回收率96%～106%，日内RSD＜6%，日间RSD＜7%.结论 本方法简单、灵敏、重现性好，适用于临床血药浓度监测，以及人体药动学的研究.

AIM To establish a method for determination of mycophenolic acid in human plasma. METHODS Mycophenolic acid was determined by HPLC using a HYPERSIL C18（ 150 mm × 5 mm,5 μm） column and a guard column ODS（5 mm × 3.9 mm, 10μm）, the mixture of methanoland and NaH2PO4（pH = 3.25） and acetonitrile（40：40：20, V/ V） as mobile phase with a flow rate of 0.8 mL·min^-1. The temperature of column was 25℃ and the wavelength of detection was 254 nm. RESULTS The standard curve was linear in the range of 0.23 - 46.13 mg· L^-1 （ r = 0. 999 7）, the method recovery was more than 96%, and the intra-day and inter-day RSD were all less than 7%. CONCLUSION This method is reproducible, convenient and sensitive for determination of plasma concentration and can be used to study clinical pharmacokinetic mycophenolic acid.