HPLC法测定头孢他啶在人椎间盘髓核的分布

Determination of ceftazidime distribution in human nucleus pulpous by HPLC

目的 建立头孢他啶血清、髓核浓度的测定方法，观察其对人体椎间盘的渗透性.方法 15例腰椎间盘突出的患者，给予头孢他啶2 g，iv gtt，30 min.给药完毕后，即行手术取髓核，同时抽取静脉血，用HPLC法测定髓核和血清中头孢他啶浓度.结果 头孢他啶为0.4～10 mg·L^-1及25～300 mg·L^-1时，药物浓度（ρ）X和药物峰面积与内标物峰面积之比（A头孢他啶/A内标）Y线性关系良好，血清标准曲线Y=4.905 1X＋0.009 2，r=0.999 9 髓核标准曲线Y=4.564 0X＋0.011 4，r=0.999 6.头孢他啶在髓核中的药量分布个体差异较大，且髓核内药量与血清药物浓度间无相关性（P＞0.05）.结论 本法可同时测定头孢他啶在血清和髓核中的药物浓度，以满足临床药物监测的需要.

AIM To establish a HPLC method for determination of ceftazidime in human plasma and nucleus pulpous, and to observe the drug penetration into human nucleus pulpous. METHODS After 2 g ceftazidime was given, iv gtt, for 30 min to 15 patients who suffered from lumbar intervertebral discitis, the nucleus pulposus specimens were obtained with operation immediately. The venous blood samples were obtained simultaneously. The ceftazidime concentration in tissues were determined by HPLC method. RESULTS The calibration curve was linear in the nucleus pulpous concentration range of 0.4 - 10 mg·L^-1 and the serum concentration range of 25 - 300 mg·L^-1 as well. The linear relation between ceftazidime concentration（p）X and the rate of peak areas （Aceftazidime/Aintemal standard）Y were all right. The serum calibration curve was Y = 4. 905 1X ＋ 0.009 2, r = 0.999 9; the nucleus calibration curve was Y = 4. 564 0X ＋ 0.011 4, r = 0. 999 6. The distribution of ceftazidime into nucleus pulpous had high interindividual variations, and the nucleus pulpous concentration did not correlate with the serum concentration （ P 〉 0.05）. CONCLUSION This method can be used to determine the serum and nucleus pulpous concentration of ceftazidime at the same time, which can satisfy the requirement of the clinical drug monitoring.