神经节苷脂GM1对D-半乳糖致衰老模型小鼠脑海马区神经发生的保护作用

The protective effect of ganglioside GM1 on the hippocampal neurogenesis in D-galactose induced mice ageing model

目的:观察神经节苷脂GM1对D-半乳糖致衰老模型小鼠脑海马神经发生的保护作用。方法:建立了D-半乳糖小鼠致衰老模型,采用免疫组织化学方法观察GM1干预对海马神经前体细胞的增殖、存活和神经元分化的作用。结果:在模型鼠海马DG区的颗粒细胞和CA1、CA3区可观察到锥形神经元的死亡,TUNEL阳性细胞位于神经前体细胞所在的颗粒细胞层和门区的交界处。GM1干预后明显保护海马区新生细胞的增殖和生存。结论:GM1通过对抗D-半乳糖导致的细胞凋亡和促进海马神经发生的途径发挥其延缓衰老的作用。

Objective：To observe the protective effect of ganglioside GM1 on hippocampal neurogenesis. Method： An established model of ageing in mice was established and immunostaining techniques was adopted to study the effect of GM1 on the proliferation, long-term survival and neuronal differentiation of hippocampal progenitors that had been injured by D-galactose. Result： D-galactose induced neuronal injury and reduced neurogenesis in hippocampal CA1,CA3 and DG subfields. TUNEL-positive neurons distributed in the juncture between granular cell layer and hilus, where neural progenitor ceils exist. GM1 treatment significantly increased the proliferation and long-term survival of hippocampal progenitor cell. Conclusion： GM1 can exert anti-ageing effect through promoting hippocampal neurogenesis.