递增负荷运动及恢复过程中大鼠雌激素受体mRNA表达的变化及其意义

Changes and significance of ER mRNA expression during progressive increasing load training and recovery periods in rats

目的:观察持续9周递增负荷运动的不同训练周期及恢复过程大鼠HPO轴各环节ER mRNA水平的变化,探讨持续运动应激致动情周期紊乱ER水平变化的机制。方法:对不同训练周期及恢复过程的大鼠,采用RT-PCR检测下丘脑、垂体、卵巢、子宫各环节ER mRNA的表达,采用液相平衡竞争放射免疫分析法检测其血清雌二醇水平。结果:训练7周结束时,垂体及下丘脑ER mRNA表达及血清E2水平均显著降低,卵巢及子宫ER mRNA表达则显著增加;HPO轴各环节ER mRNA表达的变化持续至恢复2期,血清E2水平持续至恢复3期基本复原。结论:递增负荷运动大鼠HPO轴各环节ER mRNA表达对运动负荷的反应不尽相同,ER mRNA表达的变化与血清E2水平密切相关,其表达水平的非同一性可能是运动性月经失调病理过程的重要一环。

Objective：To explore the changes of ER levelby gene expression in estruus cycle dysfunction in continuous 9 weeks exercises stress and recovery courses. Method： ER mRNA expression in each segment of HPO axis were detected by reverse transcription-polmerase chain reaction （RT-PCR）, and serum E2dynamic changes was measured by fluid equation competition RIA after 7 and 9 weeks training, 2 and 4 estrous cycles recovery courses. Result： After 7 weeks trainings ER mRNA in hypothalamus and pituitary and serum E2 level decreased markedly, ovarian and uterus ER mRNA significantly increased. ER mRNA changes of each site of HPO axis lasted to the 2nd recovery period, and the changes of serum E2 lasted to the 3th recovery period. Conclusion： After progressive increasing load training reactions of ER mRNA expression in each segment of HPO axis were different. ER mRNA expression was related to lower serum steroids concentrations, especially, E2 concentration. The different ER mRNA expression level of HPO axis may be responsible for the athletic menstrual irregularity.