不同培养液及血清浓度对绵羊孤雌生殖胚胎发育的影响

Effect of Media and Serum Concentrations on Development of Ovine Parthenogenetic Embryos

体外成熟的绵羊卵母细胞,经5μmol/L A23187 5 min和2 mmol/L 6-DMAP 4 h激活后,分别在SOFaa、M-199两种培养液中进行培养,试验比较了SOFaa、M-199液分别添加BSA和不同浓度(10%、20%)胎牛血清对绵羊孤雌生殖胚体外发育的影响。M-199+FCS组的卵裂率显著低于SOFaa+BSA和SOFaa+FCS组(P<0.05),SOFaa+BSA组的囊胚率显著低于M-199+BSA、SOFaa+FCS、M-199+FCS三组(P<0.05);SOFaa+10%FCS与SOFaa+20%FCS组的卵裂率及囊胚率均无显著差异,M-199+10%FCS和M-199+20%FCS组的卵裂率及囊胚率间均无显著差异。结果表明:SOFaa比M-199更适合绵羊孤雌生殖胚体外发育,此外添加10%的FCS即可达到较好的培养效果。

Matured ovine oocytes were activated by 5μmol/L A23187 for 5 min and then 2 mmol/L 6-DMAP for 4 h,afterwards cultured in SOFaa and M-199 medium supplemented with BSA and serum of different concentrations, respectively. The cleavage rate of group M-199＋FCS（29.9%） was significantly lower than SOFaa＋BSA（52. 7%） and SOFaa＋FCS （52.8%） groups （P〈0. 05）. And the blastocyst rate of SOFaa＋BSA（0. 8 % ） group was significantily lower than M-199 ＋BSA（7.60% ）, SOFaa＋FCS（11. 8%） ,M-199＋FCS（10.2%） groups （P〈0. 05）. The cleavage rate and blastocyst rate of oocyte treated with SOFaa＋100% FCS（50. 70%, 11.30% ） and SOFaa＋ 20 % FCS（59.20%, 9.9% ） were not significantly different, and those data of M-199＋10% FCS（29.6% ,5.6%） and M-199＋20% FCS（32. 4% ,9.9%）were also same. These results showed that SOFaa medium is suitable for the development of ovine parthenogenetic embryos cultured in vitro , and SOFaa added with10% FCS can improve its function on those IVC embryos.