精子介导法制备转基因鸡的研究

Production of Transgenic Chicken by Sperm-mediated Method

以LacZ基因为报告基因,构建载体质粒p-OV-β-Gal,转移到鸡早期胚胎内,对外源基因的整合及表达情况进行检测分析,为制备转基因鸡探索新的方法。采用脂质体介导法、精子介导法以及电激法相结合的方法将载体质粒p-OV-β-Gal转移到鸡早期胚胎内,进行转基因鸡研究,结果发现其外源基因整合阳性率为13.2%(7/53),表达阳性率为20.7%(11/53)。说明该方法可作为制备转基因鸡的有效方法。

To explore the possibility of generation of transgenic chicken, vector p-OV-β-Gal of LacZ gene as report gene were transmitted into the chicken＇s early embryos by sperm-mediated method, then the LacZ gene＇s integration and expression were monitored. The vector （p-OV-β-Gal） could be transmitted into the chicken＇s early embryos by sperm-mediated method, the percentage of LacZ gene＇s integration and expression were respectively 13. 2% （7/53）and 20. 7% （11/53）. The result showed that sperm-mediated method could be used for generation of the transgenic chickens.