子宫肿瘤恶性程度与肥大细胞脱颗粒后面积呈负相关

Negtive correlation between mast cell area ratio and the atypical degree of neoplasm from uterine smooth muscles

目的探讨肥大细胞浸润对子宫平滑肌肿瘤的影响。方法收集子宫平滑肌瘤12例、恶性未定平滑肌瘤10例和平滑肌肉瘤7例。观察和计量组织特征、肥大细胞颗粒、第Ⅷ因子相关抗原及瘤细胞凋亡率,分析肥大细胞释放颗粒与其他指标间的相关性。结果子宫平滑肌瘤瘤细胞数最少(P<0.01),恶性未定平滑肌肿瘤居中,平滑肌肉瘤最多(P<0.01)。平滑肌瘤肥大细胞脱颗粒面积百分率最多,恶性未定平滑肌瘤居中,平滑肌肉瘤最低;脱颗粒面积百分率与瘤细胞数相比呈负相关。平滑肌瘤内皮细胞第Ⅷ因子相关抗原阳性染色面积最低,恶性未定平滑肌肿瘤居中(P<0.01),平滑肌肉瘤最高(P<0.01);第Ⅷ因子相关抗原阳性染色面积与肥大细胞脱颗粒面积比呈负相关。平滑肌瘤瘤细胞凋亡率最低,恶性未定平滑肌肿瘤居中(P<0.01),平滑肌肉瘤最高(P<0.01);瘤细胞凋亡率与肥大细胞脱颗粒面积比呈负相关。结论子宫平滑肌肿瘤恶性度和血管状态与肥大细胞脱颗粒面积百分比成负相关。

Objective To investigate the effect of infiltrated mast ceils on the biological characteristics of uterine carcinoma. Methods Twelve leiomyomas, ten uncertain malignant uterine leiomyomas, and seven uterine leiomyo sarcomas were studied with light microscopy for the morphometry about their histological feature, mast cell particle and factor Ⅷ-related antigen （FⅧRAg） ; Cell apoptosis was measured by flow cytometry. The correlative analysis was carried out between the mast cell and others factors. Results Comparing with smooth muscle tumors of uncertain malignant potential （4209.9±273.0）, the count of tumor cell was the highest in leiomyosarcoma （3557.6 ± 346. 3） （P 〈 0. 01 ）, lowest in leiomyoma （ 5182. 3 ± 245.6 ） （ P 〈 0. 01 ）. The ratio of mast cell＇ s particles to whole tissue was the smallest in leiomyosarcoma （ 1.81% ± 0. 13% ） （P 〈0. 01 ）, highest in leiomyoma （4. 51% ± 0. 63% ）（P 〈0. 01 ） compared with smooth muscle tumors of uncertain malignant potential （3.41% ± 0. 16% ）. Positive FⅧRAg of endothelia was the most scant in leiomyo- sarcoma （668.98 ± 180. 87 μm^2 ） （ P 〈0.01 ）, the most abundant in leiomyoma （ 164. 50 ±57.33 μm^2） （P 〈0.01 ） compared with smooth muscle tumors of uncertain malignant potential （231.93 ± 65.37 μm^2）. The apoptosis ratio in leiomyosarcoma was the highest （15.38% ± 0. 58% ） （ P 〈 0. 01 ）, the lowest in leiomyoma （ 3.31% ± 0. 26% ） （ P 〈 0. 01 ） compared with smooth muscle tumors of uncertain malignant potential （8.36% ±0.52% ）. Negative correlation was confirmed between the ratio of mast cell＇ s particles to whole tissue and the cell count, the ratio of particles in mast cells, or the apoptotic ratio in all uterine tumors. Conclusion A negtive correlation between the remained area ratio of mast cell and the atypical degree of neoplasm from uterine smooth muscles is confirmed.