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NGF及其受体P75在四氯化碳中毒性大鼠肝组织中表达增强 被引量：6

Increased expressions of NGF and its receptor P75 in liver of CCl_4-induced toxic rats
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摘要 目的观察四氯化碳中毒时神经生长因子(NGF)及其受体P75在大鼠肝组织的表达变化,探讨NGF在肝纤维化发生机制中的可能作用。方法用四氯化碳致大鼠肝中毒模型,用RT-PCR法和Western blot法,分别检测NGF及其受体P75在大鼠肝组织中的表达。结果与对照组大鼠肝组织中NGFmRNA及其受体P75蛋白表达量比较,造模组24 h显著增高(P<0.01,n=6),造模48 h和72 h迅速回降,但仍显著高于对照组(P<0.05,n=6)。结论在四氯化碳中毒性大鼠的肝组织中,NGF及其受体P75的表达可能与肝纤维化的形成密切相关。 Objective By observing the changes in NGF and its receptor P75 in liver of CCl4-induced toxic rats and to evaluate the role of NGF in the mechanism of hepatic fibrosis. Methods The expressions of NGF mRNA and its receptor P75 were detecteded by both hemi-quantitative RT-PCR and Western blot analysis. Results The expressions of NGF mRNA and its receptor P75 in liver of CCl4-induced toxic rats at 24th hour group were higher than that in control group（P 〈0. 01 ,n =6）. Then it got down at 48th hour and 72th hour. But it was still higher than that of control group（P 〈 0. 05, n = 6）. Conclusion In CCl4-induced rat liver tissue, the expressions of NGF and its receptor P75 is potentially related to hepatic fibrosis.
作者 陈新 姜慧卿 贺战国 王玉珍 扈彩霞
机构地区 河北医科大学第二医院消化科 白求恩国际和平医院中心实验室
出处 《基础医学与临床》 CSCD 北大核心 2007年第7期802-805,共4页 Basic and Clinical Medicine
关键词 NGF RT-PCR WESTERNBLOT 肝纤维化 大鼠 NGF RT-PCR Western blot hepatic fibrosis rat
分类号 R595.1 [医药卫生—内科学]
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参考文献10

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二级参考文献35

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基础医学与临床
2007年 第7期

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