梅毒血清学五种检测方法的比较

The Comparative Study of Five Serologic Tests for Syphilis

目的比较五种梅毒血清学检测方法对各期梅毒血清标本进行检测的结果,评价各种方法的临床适用性。方法用酶联免疫吸附试验(ELISA)、胶体金快速检测试验(SYP)、梅毒螺旋体血凝试验(TPHA)、梅毒螺旋体明胶颗粒凝集试验(TPPA)做特异性抗体定性检测;甲苯胺红不加热血清试验(TRUST)做非特异性抗体半定量检测。结果ELISA,SYP,TPHA,TPPA四种方法对梅毒特异性抗体的定性检测结果差异无显著性;TRUST对二期梅毒非特异性抗体检出率最高,其余各期阳性率相对较低。结论①使用SYP对临床上疑诊梅毒的患者进行筛选,结合TRUST的实验结果可对梅毒进行诊断。②综合考虑梅毒特异性抗体和非特异性抗体的滴度变化水平才能真实评价治疗效果。

Objective Comparing the result of five syphlis serologic tests to each stage syphlilis serum specimens , and evaluating each methods. Methods The ELISA, SYP, TPPA, TPHA were used to make qualitative detection of specific antibody, and TRUST was used to make titer detection of non-specific antibody. Results The qualitative detection to specific antibody of syphilis in the TPHA,TPPA,ELISA and SYP had no statistic difference;the postive ratio in the TRUST was higher to secondary stage syphilis,but lower to the other stages, Conclusion （1）Using the SYP to screen the clinical presumptive syphilis, and with the result of TRUST could diagnose syphilis at different stages. （2）The treatment effect could be truly evaluated by considering comprehensively the titer＇ s change of specific and non-specific antibodies to TP,