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毕赤酵母表达系统 被引量：3

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摘要 毕赤酵母表达系统是一种优秀的重组蛋白表达系统,近年来被广泛应用于重组蛋白的表达。现就巴斯德毕赤酵母表达系统的特点及其用于外源蛋白表达的基本策略作一综述。
作者 彭彦孟（综述） 连继勤（审校）
机构地区 第三军医大学学员旅学员 第三军医大学生物化学与分子生物学教研室
出处 《国际检验医学杂志》 CAS 2007年第7期638-640,共3页 International Journal of Laboratory Medicine
关键词 毕赤酵母 重组蛋白
分类号 R379 [医药卫生—病原生物学]
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参考文献13

  • 1Cereghino GP, Cereghino JL, Ilgen C, et al. Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris[J]. Curr Opin Biotechnol, 2002, 13(4) : 329-332.
  • 2Cereghino JL, Cregg JM. Heterologous protein expression in the methy-lotrophie yeast Piehia pastoris [J]. FEMS Mierobiol Rev, 2000, 24(1); 45-66.
  • 3Li Z, Xiong F, Lin Q, et al. Low-temperature increases the yield of- biologieally active herring antifreeze protein in Piehia pastoris[J]. Protein Expr Purif, 2001, 21(3): 438-445.
  • 4Daly R, Hearn MT Expression of heterologous proteins in Pichia pastorls: a useful experimental tool in protein engineering and production [J]. J Mol Recognit, 2005,18(2) : 119-138.
  • 5Macauley PS, Fazenda ML, McNeil B, et al. Heterologous protein production using the Pichia pastoris expression system [J]. Yeast, 2005, 22(4): 249-270.
  • 6Ciofalo V, Barton N, Kreps J, et al. Safety evaluation of a lipase enzyme preparation, expressed in Pichia pastoris, intended for use in the degumming of edible vegetable oil[J]. Regul Toxicol Pharmacol, 2006, 45(1): 1-8.
  • 7聂东宋,梁宋平,李敏.外源蛋白在巴氏毕赤酵母中高效表达的策略[J].吉首大学学报,2001,22(3):40-44. 被引量：19
  • 8Ohya T, Ohyama M, Kobayashi K. Optimization of human serum albumin production in methylotrophlc yeast Pichia pastoris by repeated fed-batch fermentation[J]. Bioteehnol Bioeng, 2005, 90(7): 876-87.
  • 9Kumita JR, Johnson RJ, Alcocer MJ, et al. Impact of the native-state stability of human lysozyme variants on protein secretion by Pichia pastoris[J]. J FEBS, 2006, 273(4) : 711-720.
  • 10Lee DN, Kuo TY, Chen MC, et al. Expression of porcine epidermal growth factor in Pichla pastoris and its biology activity in early-weaned piglets[J]. Life-Sci, 2006, 78(6): 649-54.

二级参考文献26

  • 1王洪海,高卜渝,陈佩丽,董灵,李育阳.酵母表达基因工程产物不均一性分析及其对策[J].中国科学（B辑）,1994,24(12):1270-1274. 被引量：12
  • 2唐南筠,霍克克,李育阳.乳酸克鲁维酵母高拷贝整合载体的构建及应用[J].生物化学与生物物理学报,1996,28(5):540-546. 被引量：10
  • 3GREGG J M, TSCHOPP J F, STILLMAN C, et al. High-level Expression and Efficient Assembly of Hepatitis B Surface Antigen in the Methy Lotrophic Yeast Pichia Pastoris[J]. Bio/Technology, 1987, (5):479-486.
  • 4MC GREW J T, LEISKE D, DELL B, et al. Expression of Trimeric CD40 Ligand in Pichia Pastoris: Use of a Rapid Method to Detect High-level Expression Trarsformants[ J]. Gene, 1997, ( 187 ): 193-198.
  • 5CLARE J J, ROMANOS M A. Production of EGF in Yeast:High-level Secretion Using Pichia Pastoris Strains Containing Multiple Gene Copies[J]. Gene, 1991,105:205-212.
  • 6MEI M, WHITTAKER, JAMESW, et al. Expression of Recombinant Galactose Oxidase by Pichia Pastoris[ J]. Protein Expression and Purification,2000,20:105-111.
  • 7CREGG J M,UDEVICK T S, RASCHKE W C. Recent Advances in the Expression of Foreign Genes in Pichia Pastoris[J]. Bio/Technology, 1993,11 (8) :905-910.
  • 8CLARE J J, RAYMENT F B, BALLANTINE S P, et al. Hihg-level Expression of Tetanus Toxin Fragment C in Pichia Pastoris Strains Containing Multiple Tandem Integration of the Gene[J]. Bio/Technology, 1991, (9):455-460.
  • 9MICHAEL J D. Over Expression in Pichia Pastoris and Crystallization of an Elicitor Protein Secreted by the Phytopathogenic Fungus[J]. Protein expression and Purification, 1996,(8):254-261.
  • 10LOEVEN M C. Biosynthetic Production of Type Ⅱ Fish Antifreeze Protein: Fermentation by Pichia Pastris[ J ]. Appl Microbiol Bitechnol, 1997,(48):480-486.

共引文献18

同被引文献21

引证文献3

二级引证文献1

国际检验医学杂志
2007年 第7期

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