慢性酒精中毒致大鼠学习记忆障碍模型

Rat model of learning and memory disorder induced by chronic alcoholism

目的:建立慢性酒精中毒致大鼠学习记忆障碍模型。方法:用55%酒精给大鼠灌胃6周,同时运用Morris水迷宫检测大鼠学习记忆损伤情况,气相色谱法检测大鼠血液酒精浓度,并观察酒精染毒后大鼠海马组织的病理学变化。结果:酒精灌胃6周,大鼠海马病理学变化、学习记忆损伤程度及病死率,以高浓度染毒组(HAA组)最明显,中浓度染毒组(MAA组)次之,低浓度染毒组(LAA组)不明显,且血液酒精浓度与学习记忆损伤程度的呈直线相关。结论:以每只大鼠每日10 ml/kg酒精灌胃6周,可初步建立慢性酒精中毒致大鼠学习记忆障碍模型。

Objective ：To establish the rat model of learning and memory disorder induced by chronic alcoholism. Methods： The rat model of learning and memory disorder was established by intragastric administration of 55% alcohol for 6 weeks to detect the changes of learning and memory by Morris water maze and blood alcohol concentration by gas chromatography for observing pathological changes of hippocampus. Results： After 6 weeks＇ intragastric administration,dominant pathological changes in hippocampus , impairment of learning and memory and death rates were observed in the high alcohol administration group（HAA group）, arid that was less serious in the moderate alcohol administration group（MAA group）. There was no obvious changes detected in the low alcohol administration group（ LAA group）. Blood alcohol concentration showed rectilinear correlation with the impairment of learning and memory. Conclusion ： 10 ml/kg alcohol administered to a rat daily for 6 weeks can be preliminary establishment of the rat model for studying learning and memory disorder.