外源性血管内皮生长因子在激光诱导脉络膜视网膜静脉吻合中的作用

The Effects of Exogenous VEGF on the Course of Laser-induced Chorioretinal Venous Anastomosis

目的分析外源性血管内皮生长因子(vascular endothelial growth factor,VEGF)对提高激光诱导脉络膜视网膜静脉吻合(chorioretinal venous anastomosis,CRVA)形成成功率的影响。方法应用激光在兔眼建立视网膜静脉阻塞(retinal vein occlusion,RVO)和 CRVA 模型,根据外源性药物干预浓度设3个实验组,2周后经玻璃体注射已设计的不同浓度、剂量的外源性 VEGF,于4～5周行眼底血管荧光造影检查,判断 CRVA 是否形成。另设空白对照组与阴性对照组。结果实验干预组 CRVA 成功率分别为31.25%,43.75%和43.75%,对照组 CRVA 成功率分别为31.25%和37.50%。5组成功率差异无统计学意义(P>0.05)。结论在本实验条件下,外源性 VEGF 对激光诱导 CRVA 形成无明显促进作用。(中国眼耳鼻喉科杂志,2007,7:214-216)

Purpose To explore if the exogenous vascular endothelial growth factor（VEGF） could increase the successful rate of the formation of laser - induced chorioretinal venous anastomosis（CRVA）. Methods Retinal vein occlusion（RVO） in rabbit eyes was created and the CRVA was induced by laser radiation. The rabbits then were divided into 3 test groups based on the different concentration of VEGF received and 2 control groups（ sham and negative）. Two weeks later, the rabbits of the test groups received different designed doses of exogenous VEGF by intravitreous injection, the rabbits of the negative control received venicle solution. Within four to five weeks, the CRVA formation was checked by fundus fluorescein angiography （FFA）. Results The successful rate of CRVA formation was 31.25%, 43.75%, 43.75% in the test groups, and 31.25%, 37.50% in the control groups respectively. There was no significant difference among five groups （P 〉 0.05）. Conclusion In the present study, the exogenous VEGF has no effect in promoting the laser- induced chorioretinal venous anastomosis.