玉米秸秆超声辅助酶水解

Study on Ultrasound Assisted Enzymatic Hydrolysis of Corn Stover

利用超声波技术研究了外加超声场条件下玉米秸秆的纤维素酶水解过程。结果表明:超声波可有效地提高玉米秸秆的纤维素酶水解得率,减少酶用量。在超声频率20kHz、功率30W、作用时间10min的超声场下,纤维素酶的最适滤纸酶活用量为20IU/g,最适水解温度为50℃,最适pH为4.8,其48h酶解得率达到27.3%,比未加超声波时酶解得率提高了48.3%。

The enzymatic hydrolysis of corn stover under ultrasound was studied. The results showed that the rate of hydrolysis could be enhanced by ultrasound. The effects of ultrasound power, irradiation time, temperature, enzymedosage, and pH were discussed in detail. Under the condition of 20 kHz, 30 W power and 10 min irradiation time,the optimal enzyme dosage was 20 IU/g dry substrate. The optimal temperature was 50℃ and the optimal pH was 4. 8. The hydrolysis yield reached 27.3% after 48 h and was 48.30/oo higher than that without ultrasound.