抗松材线虫病赤松家系的抗性测定及组织病理学观察

Resistance Determination and Histopathological Observation of Induced Pinus densiflora Families from Japan to Bursaphelenchus xylophilus

对自日本引进的赤松(Pinus densiflora)27个抗病家系进行人工抗病性测定,结果发现抗病各家系对3个不同来源的松材线虫(Bursaphelenchus xylophilus)虫株都表现出不同程度的抗病性,且差异明显。综合3次接种试验结果发现,引进赤松中有13个家系(1、4、5、6、7、8、13、14、15、17、20、23和25号)抗病表现较好。对5号抗病家系接种松材线虫后,分别按时间进程(4、8、12、24、36、48、72、96、120h)进行茎段取样,组织病理学观察发现松材线虫在引进的抗病赤松体内的迁移速度较普通赤松慢,且松树茎内木射线细胞和树脂道泌脂细胞周围的薄壁细胞出现病变较普通赤松晚,病变程度也比普通赤松轻。

The resistance of 27 wilt-resistant Pinus densiflora families induced from Japan to 3 isolates of Bursaphelenchus xylophilus wa＇s determinated. The result indicated that all 27 Pinus densiflora families had resistance to the 3 isolates, and there were significant differences from each other.It was found by bark inoculation that 13 families（No. 1,4,5,6,7,8, 13,14,15,17,20,23 and 25） were more resistant than others. The histopathological observation of No. 5 family sampled at 4,8,12,24,36,48,72,96,120 h after inoculation showed that B. xylophilus migrated much slower in the induced pine seedlings than in the control. Pathological changes in parenchyma radial cell and the parenchymatous cells around resinosis cells occured later in wilt-resistant pine seedlings than in the control. In addition, the pathological changes occured more gently in the induced pine seedlings than in the control.