摘要
采用引物对K09F2/R对枯死松树内分离的松材线虫、拟松材线虫及其他线虫进行检测,结果表明,所有松材线虫株系均扩增出一条340bp的清晰、明亮的条带。而拟松材线虫、其他线虫均无扩增产物。利用引物对K09F2/R构建了松材线虫检测试剂盒。采用该试剂盒可使整个检测过程不超过2.5h;该试剂盒检测灵敏度为1/7条线虫,达到了对幼虫成功鉴定的目的,且检测结果便于判读,检测费用较低。
Bursaphelenchus xylophilus, B. rnucronatus and the other nematodes separated from dead pine .were detected by using primer K09F2/R. The results indicated that the PCR product of all B. xylophilus isolates had a clear and bright fragment about 340bp. But B. mucronatus and other nematodes had not any fragments. So pairwise K09F2/R was used for constructing identification kit of B. xylophilus. It took a short time to identify nematodes by adopting this kit. The time of total detection process was within two and half hours. Besides, the sensitivity of this kit reached to 1/7 of a nematode. The detection result is easy to estimate, and the cost of detection is quite low. This kit is a rapid, reliable and convenient means for detecting B. xylophilus.
出处
《南京林业大学学报（自然科学版）》
CAS
CSCD
北大核心
2007年第4期133-135,共3页
Journal of Nanjing Forestry University：Natural Sciences Edition
基金
安徽省林业厅与南京林业大学合作研究项目(2002-2005)
江苏省博士后基金计划项目(2005-2007)
关键词
松材线虫
快速检测
试剂盒
Bursaphelenchus xylophilus
Quick detection
Kit
