黄芪四君汤对脾虚证大鼠甲状腺素及环核苷酸水平的影响

Effects of Huangqi Sijun Decoction on Thyroxin and Cyclic Nucleotide Levels in Rat Models with Spleen Deficiency Syndrome

目的观察黄芪四君汤对脾虚证模型大鼠甲状腺激素及环核苷酸水平的影响,探讨该方防治脾虚证的作用机理。方法SD雄性大鼠随机分为3组:正常对照组,脾虚模型组,黄芪四君汤组。采用大黄复制脾虚证动物模型,观察各组动物血清甲状腺素(T4)、三碘甲腺原氨酸(T3)、促甲状腺素(TSH)及血浆环磷酸腺苷(cAMP)、环磷酸鸟苷(cGMP)水平的变化。结果与正常对照组比较,脾虚模型组大鼠血清T3、T4含量下降(P<0.01),血浆cAMP含量及cAMP/cGMP比值均升高(P<0.01)。黄芪四君汤能提高实验性脾虚证大鼠T3、T4水平及脾脏指数、胸腺指数,降低cAMP含量及cAMP/cGMP比值。结论黄芪四君汤能改善脾虚证模型大鼠的脾虚症状,其作用机理与调节甲状腺激素及cAMP、cGMP水平有关。

Objective To investigate the effects of Huangqi Sijun Decoction on the levels of thyroxin and cAMP and cGMP in model rats with spleen deficiency syndrome and to explore its therapeutic mechanism. Methods Male SD rats aged three months were randomly divided into 3 groups： normal control group, spleen deficiency model group and Huangqi Sijun Decoction group. The rat models of spleen deficiency syndrome were established by gavage with the decoction of Rhubarb. The serum levels of thyroxin （T4）, 3, 5, 3＇ - triiodothyronine （T3）, thyrotropin （TSH） and plasma levels of cAMP, cGMP were determined. Results T3 and T4 were significantly lower but the levels of cAMP and cAMP/cGMP were significantly higher in the spleen deficiency models than those in the normal control group （ P 〈 0. 01 ） . After treatment with Huangqi Sijun Decoction, the levels of %, T4, the body weight and weight ratio of thymus and spleen in the spleen deficiency models were increased, while the levels of cAMP and cAMP/cGMP were decreased. Conclusion Huangqi Sijun Decoction can relieve the symptoms of spleen deficiency in rat models, and its mechanism is related to the regulation of thyroxin, cAMP and cGMP levels.