摘要
目的建立用液相色谱法测定紫肉甘薯中氯化花青素含量的方法。方法高效液相色谱法,用Kromasil C18柱(4.6mm×250mm,5μm);流动相:乙腈-水-冰醋酸(15∶76.5∶8.5);紫外检测波长:530nm。结果建立了高效液相色谱法测定氯化花青素含量的方法;氯化花青素的线性范围为0～0.2089μg(r=0.9998);平均回收率为97.95%,RSD=1.0%。结论方法简单、快速,结果准确。
Objective To develop a HPLC method for the determination of anthocyanin chloride in purple sweet pota-to. Methods The analysis was carried out by using Kromsail consists of acetonitrile- water- acetic acid （15 ： 76.5 ： 8.5 ）. C18 column（4.6 mm× 250 mm, 5 μm）. The mobile phase The wavelength of detection was at 530 nm. Results The linear range of anthocyanin was 0 - 0. 208 9 μg（ r = 0. 999 8）. The average recovery was 97.95 % （RSD = 1.0 % , n = 5）. Conclusions This method is simple and reliable.
出处
《中药新药与临床药理》
CAS
CSCD
2007年第4期312-313,共2页
Traditional Chinese Drug Research and Clinical Pharmacology
